Multiple Displacement Amplification:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
Recombinant:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
shRNA:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
Positive Control:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
Sequencing:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
Negative Control:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
CRISPR:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MCF10A PTEN+/+ cells Horizon Cat#HD 101-006 MCF10A PTEN / cells Horizon Cat#HD 101-006 JIMT-1 cells DSMZ Cat#ACC 589 HCT116 parental cells ATCC Cat#CCL-247 293T ATCC CatCRL-3216 PC-3 ATCC Cat#CRL-1435 H460 ATCC Cat#HTB-177 H1299 ATCC Cat#CRL-5803 U-138 MG ATCC Cat#HTB-16 MDA-MB-134 ATCC Cat#HTB-23 BT-474 ATCC Cat#HTB-20 UACC-812 ATCC Cat#CRL-1897 MCF7 ATCC Cat#HTB-22 Hs 578T ATCC Cat#HTB-126 SK-BR-3 ATCC Cat#HTB-30 AU565 ATCC Cat#CRL-2351 BT-20 ATCC Cat#HTB-19 ZR-75-30 ATCC Cat#CRL-1504 Experimental Models: Organisms/Strains Mouse: C57BL/6J Charles River Stock No: 000664 Mouse: Pten+/ Di Cristofano et al., 1998 N/A Mouse: Park2 / Itier et al., 2003 N/A Mouse: NOD.CB17-Prkdcscid/J Charles River Stock No: 001303 Recombinant DNA TRC Lentiviral eGFP shRNA positive control Dharmacon Cat#RHS4459 TRC Human PARK2 shRNA 1 Dharmacon Cat# TRCN0000000283 TRC Human PARK2 shRNA 2 Dharmacon Cat# TRCN0000000285 pLenti6/UbC/V5-DEST-empty vector ThermoFisher Cat#V49910 pLenti6/UbC/V5-DEST-PARK2-WT This study N/A pLenti6/UbC/V5-DEST-PARK2-C431S This study N/A pLV430G-oFL-T2A-eGFP Green et al., 2015 N/A pRK5-Myc-PTEN-WT Song et al., 2011 N/A pRK5-Myc-PTEN-C83S This Study N/A HA-Ubiquitin Addgene Cat#18712 pHR-SIN-PTEN-WT Addgene Cat#30370 pHR-SIN-PTEN-C83S This Study N/A Sequence-Based Reagents Silencer Select Non-targeting Negative Control ThermoFisher Cat#4390843 Silencer Select Pre-Designed siRNA against PRKAA1 (AMPKa1): siRNA ID: s100 ThermoFisher Cat#4392420 Silencer Select Pre-Designed siRNA against PRKAA2 (AMPKa2): siRNA ID: s11056 ThermoFisher Cat#4390824 LentiCRISPR v2 Addgene Cat#52961 PARK2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) Applied Biological Materials Cat#K1594608 (Continued on next page) e3 Molecular Cell 65, 999–1013.e1–e7, March 16, 2017 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
other:Article Title: PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers for human PARK2 (F: GGTAGATCAATCTACAACAGCTTTTATG, R: TGCACTAGTCCCAGGGCA) Hedrich et al., 2001 N/A Primers for human PTEN (F: CAAGATGATGTTTGAAACTATTCCAATG, R: CCTTTAGCTGGCAGACCACAA) Kim et al., 2004 N/A Primers for human 18S (F: GTGGAGCGATTTGTCTGGTT, R: CGCTGAGCCAGTCAGTGTAG) Leonard et al., 2003 N/A Software and Algorithms R statistical software (ver 3.2.3) and Bioconductor (ver 3.4) The R Project https://www.r-project.org/ https://www.bioconductor.org/ Oncomine (ver 4.5) ThermoFisher https://www.oncomine.org/ GISTIC Broad Institute http://portals.broadinstitute.org/tcga/home MultiQuant (ver 1.1) SCIEX https://sciex.com/products/software/ multiquant-software KM plotter Szász et al., 2016 http://kmplot.com/analysis/
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